한빛사 논문
Jae Ryul Baea,1, Wongyoung Leea,1, Young Ok Joa, Sukmin Hana, Soulmee Koha, Woo Keun Songc, Sung Hyun Kimb,d,*
aDepartment of Neuroscience, Graduate School, Kyung Hee University, Seoul, 02447, South Korea
bDepartment of Physiology, School of Medicine, Kyung Hee University, Seoul, 02447, South Korea
cSchool of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, South Korea
dMedical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Seoul, 02447, South Korea
1These authors contributed equally.
*Corresponding author
Abstract
Proper brain function requires a balance between excitatory and inhibitory neuronal activity. This balance, which is disrupted in various neural disorders, ultimately depends on the functional properties of both excitatory and inhibitory neurons; however, how the physiological properties of presynaptic terminals are controlled in these neurons is largely unknown. In this study, we generated pHluorin-conjugated, synaptic vesicle-specific tracers that are preferentially expressed in excitatory or inhibitory nerve terminals. We found that synaptic vesicle recycling is ∼1.8-fold slower in inhibitory nerve terminals than excitatory nerve terminals, resulting in reduced efficacy of synaptic transmission in inhibitory presynaptic terminals during repetitive activities. Interestingly, this relative difference in trafficking efficiency is mediated by synaptic vesicle protein 2A (SV2A), which is more highly expressed in inhibitory synapses and differentially controls sorting of synaptic protein, synaptotagmin I. These findings indicate that SV2A coordinates distinct properties of synaptic vesicle recycling between excitatory and inhibitory synapses.
Keywords : SV2A; Excitatory synapse; Inhibitory synapse; Synaptic vesicle recycling; Synaptic transmission; E/I balance
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