한빛사 논문
Hye Sung Kima, Nandin-Erdene Mandakhbayarb,c, Hae-Won Kimb,c, Kam W. Leongd, Hyuk Sang Yooa,e,*
aDepartment of Biomedical Materials Engineering, Kangwon National University, Chuncheon, 24341, Republic of Korea
bInstitute of Tissue Regeneration Engineering, Dankook University, Cheonan, 330-714, Republic of Korea
cDepartment of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea
dDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA
eInstitute of Bioscience and Biotechnology, Kangwon National University, Republic of Korea
*Corresponding author
Abstract
Cartilage defect is difficult to heal due to its avascular properties. Implantation of mesenchymal stem cell is one of the most promising approach for regenerating cartilage defects. Here we prepared polymeric nanofibrils decorated with cartilage-derived decellularized extracellular matrix (dECM) as a chondroinductive scaffold material for cartilage repair. To fabricate nanofibrils, eletrospun PCL nanofibers were fragmented by subsequent mechanical and chemical process. The nanofibrils were surface-modified with poly(glycidyl methacrylate) (PGMA@NF) via surface-initiated atom transfer radical polymerization (SI-ATRP). The epoxy groups of PGMA@NF were subsequently reacted with dECM prepared from bovine articular cartilage. Therefore, the cartilage-dECM-decorated nanofibrils structurally and biochemically mimic cartilage-specific microenvironment. Once adipose-derived stem cells (ADSCs) were self-assembled with the cartilage-dECM-decorated nanofibrils by cell-directed association, they exhibited differentiation hallmarks of chondrogenesis without additional biologic additives. ADSCs in the nanofibril composites significantly increased expression of chondrogenic gene markers as well as osteogenic ones in comparison to those in pellet culture. Furthermore, ADSC-laden nanofibril composites filled the osteochondral defects compactly due to their clay-like texture. Thus, the ADSC-laden nanofibril composites supported the long-term regeneration of 12 weeks without matrix loss during joint movement. The defects treated with the ADSC-laden PGMA@NF significantly facilitated reconstruction of their cartilage and subchondral bone ECM matrices compared to those with ADSC-laden nanofibrils, non-specifically adsorbing cartilage-dECM without surface decoration of PGMA.
Keywords : Electrospinning; Nanofiber; Atom transfer radical polymerization; Decellularization; Cartilage repair
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