한빛사 논문
Kyung Oh Jung1, Tae Jin Kim1, Jung Ho Yu2, Siyeon Rhee3, Wei Zhao1, Byunghang Ha1, Kristy Red-Horse3, Sanjiv Sam Gambhir2, Guillem Pratx1,*
1Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA, USA.
2Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA.
3Department of Biology, Stanford University School of Medicine, Stanford, CA, USA.
*Corresponding author
Abstract
In vivo molecular imaging can measure the average kinetics and movement routes of injected cells through the body. However, owing to non-specific accumulation of the contrast agent and its efflux from the cells, most of these imaging methods inaccurately estimate the distribution of the cells. Here, we show that single human breast cancer cells loaded with mesoporous silica nanoparticles concentrating the 68Ga radioisotope and injected into immunodeficient mice can be tracked in real time from the pattern of annihilation photons detected using positron emission tomography, with respect to anatomical landmarks derived from X-ray computed tomography. The cells travelled at an average velocity of 50 mm s−1 and arrested in the lungs 2–3 s after tail-vein injection into the mice, which is consistent with the blood-flow rate. Single-cell tracking could be used to determine the kinetics of cell trafficking and arrest during the earliest phase of the metastatic cascade, the trafficking of immune cells during cancer immunotherapy and the distribution of cells after transplantation.
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