한빛사 논문
Donghyun Ka1,4, Hyejin Oh1,2,4, Eunyoung Park1, Jeong-Han Kim1,3 & Euiyoung Bae1,3,*
1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea. 2Department of Applied Biology and Chemistry, Seoul National University, Seoul 08826, Korea.
3Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
4These authors contributed equally: Donghyun Ka, Hyejin Oh
*Corresponding author
Abstract
The intense arms race between bacteria and phages has led to the development of diverse antiphage defense systems in bacteria. Unlike well-known restriction-modification and CRISPR-Cas systems, recently discovered systems are poorly characterized. One such system is the Thoeris defense system, which consists of two genes, thsA and thsB. Here, we report structural and functional analyses of ThsA and ThsB. ThsA exhibits robust NAD+ cleavage activity and a two-domain architecture containing sirtuin-like and SLOG-like domains. Mutation analysis suggests that NAD+ cleavage is linked to the antiphage function of Thoeris. ThsB exhibits a structural resemblance to TIR domain proteins such as nucleotide hydrolases and Toll-like receptors, but no enzymatic activity is detected in our in vitro assays. These results further our understanding of the molecular mechanism underlying the Thoeris defense system, highlighting a unique strategy for bacterial antiphage resistance via NAD+ degradation.
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기