한빛사 논문
Abstract
Jin-Hee Kim, Se-Wook Oh*
Department of Foods and Nutrition, Kookmin University, Seoul 136-702, Korea
*Corresponding author
Abstract
The outbreaks due to the low number of foodborne pathogens present in ready-to-eat products can be prevented by rapid and sensitive detection method. However, as a conventional detection method, it is impossible to monitor foodborne bacteria existing which is less than 50 cfu/25 g in a food. This study was designed to investigate the possibility of detecting 1 cfu in the short-term through filtration, DNA concentration, and qPCR. As a result of the filtration + DNA concentration method, the recovery concentrations of Escherichia coli O157:H7 and Salmonella Typhimurium was not significantly different from initial inoculation (> 7 cfu/25 g). In iceberg lettuce and cabbage, this method was able to detect 7 and 7 cfu/25 g of E. coli and 68 and 5 cfu/25 g of S. Typhimurium. We demonstrated the potential of the filtration + DNA concentration method as a shorter time alternative to conventional enrichment-based rapid detection in vegetables.
Keywords
Filtration; rapid detection; real-time PCR; Escherichia coli O157:H7; without enrichment; 2-h detection
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