한빛사 논문
Eun-Jin Parka,b,c, Jongsik Chund, Chang-Jun Chae, Wan-Soo Parkf, Che Ok Jeong,*, Jin-Woo Baea,b,*
aDepartment of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea
bDepartment of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea
cDepartment of Food Bioengineering, Jeju National University, Jeju 690-756, Republic of Korea
dSchool of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea
eDepartment of Biotechnology (BK21 Program), Chung-Ang University, Anseong 456-756, Republic of Korea
fWorld Institute of Kimchi, Seongnam 463-746, Republic of Korea
gDepartment of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea
*Corresponding author
Abstract
Kimchi, a food made of fermented vegetables, is densely populated by indigenous microorganisms that originate from the raw ingredients under normal conditions. Most microbiological studies on kimchi have been on the most popular dish, baechu-kimchi (Chinese cabbage kimchi). Therefore, relatively little is known about the various other kinds of kimchi (depending on the region, season, main ingredient, starter culture inoculation and recipe). In this study, we collected 100 samples periodically during the fermentation of ten representative kinds of kimchi (including starter-inoculated kimchi) that were stored in the refrigerator (4 °C) during the 30−35 days fermentation period. The multiplex barcoded pyrosequencing of a hypervariable V1−V3 region of the 16S ribosomal RNA (rRNA) gene tagged with sample-specific barcodes for multiplex identifiers was employed for bacterial community profiling. We found that bacterial communities differed between starter-inoculated and non-inoculated kimchi at the early stages of fermentation, but overall there were no significant differences in the late phases. Also, the diversity and richness of bacterial communities varied depending on the various types of kimchi, and these differences could largely be explained by the major ingredients and the manufacture processes of each types of kimchi. This study provides the comprehensive understanding of the factors influencing the biodiversity of the kimchi ecosystem.
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