한빛사 논문
경상대학교
Abstract
Ju Hye Janga, Hyang Mi Leeb, Hyun Kima, Ju Hyun Choa,b,c,*
aResearch Institute of Life Science, Gyeongsang National University, Jinju, 52828, South Korea
bDivision of Applied Life Science, Gyeongsang National University, Jinju, 52828, South Korea
cDivision of Life Science, Gyeongsang National University, Jinju, 52828, South Korea
*Corresponding author
Astract
Deubiquitinase cylindromatosis (CYLD) inhibits MAPK and NF-κB activation pathways by deubiquitinating upstream regulatory factors. Although CYLD has been identified and actively studied in mammals, nothing is known about its putative function in fish. In this study, we identified the gene encoding CYLD (OmCYLD) from rainbow trout, Oncorhynchus mykiss, and examined its role during pathogenic infections. The deduced amino acid sequence of OmCYLD contains conserved CAP-Gly and USP domains. In RTH-149 cells, the expression of OmCYLD was increased by stimulation with Edwardsiella tarda and Streptococcus iniae. Gain-of-function and loss-of-function experiments showed that OmCYLD down-regulates the activation of MAPK and NF-κB and the expression of pro-inflammatory cytokines in E. tarda-stimulated RTH-149 cells. These findings suggest that OmCYLD might function like those of mammals to negatively regulate bacteria-triggered signaling pathway in fish.
Keywords
CYLD; Deubiquitinase; Innate immunity; Teleost; Rainbow trout; RTH-149
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