한빛사 논문
Youn Jae Jung1,2,*, Hark Kyun Kim3,*, Yoonsuk Cho3, Ji Suk Choi1,2, Chang Hee Woo1,2, Kyoung Soo Lee1, Jae Hoon Sul3, Chan Mi Lee2, Jihoon Han3, Jae Hyung Park2,4,5, Dong-Gyu Jo2,3,5,† and Yong Woo Cho1,2,†
1Department of Materials Science and Chemical Engineering, Hanyang University ERICA, Ansan 15588, Republic of Korea.
2ExoStemTech Inc., Ansan 15588, Republic of Korea.
3School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.
4School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
5Biomedical Institute for Convergence, Sungkyunkwan University, Suwon 16419, Republic of Korea.
†Corresponding author : (D.-G.J.), (Y.W.C.)
*These authors contributed equally to this work.
Abstract
Stem cell–derived extracellular vesicles (EVs) offer alternative approaches to stem cell–based therapy for regenerative medicine. In this study, stem cell EVs derived during differentiation are developed to use as cell-free therapeutic systems by inducing tissue-specific differentiation. EVs are isolated from human adipose-derived stem cells (HASCs) during white and beige adipogenic differentiation (D-EV and BD-EV, respectively) via tangential flow filtration. D-EV and BD-EV can successfully differentiate HASCs into white and beige adipocytes, respectively. D-EV are transplanted with collagen/methylcellulose hydrogels on the backs of BALB/c mice, and they produce numerous lipid droplets in injected sites. Treatments of BD-EV attenuate diet-induced obesity through browning of adipose tissue in mice. Furthermore, high-fat diet–induced hepatic steatosis and glucose tolerance are improved by BD-EV treatment. miRNAs are responsible for the observed effects of BD-EV. These results reveal that secreted EVs during stem cell differentiation into white adipocytes or beige adipocytes can promote cell reprogramming.
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