한빛사 논문
My Kieu Ha1,2, Sook Jin Kwon1,3, Jang‐Sik Choi1,3, Nguyen Thanh Nguyen1,2, Jaewoo Song4, Yangsoon Lee5, Young‐Eun Kim5, Incheol Shin6, Jin‐Wu Nam6 and Tae Hyun Yoon1,2,3,*
1Center for Next Generation Cytometry, Hanyang University, Seoul, 04763, Republic of Korea.
2Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
3Institute of Next Generation Material Design, Hanyang University, Seoul, 04763, Republic of Korea.
4Department of Laboratory Medicine, College of Medicine, Yonsei University, Seoul, 03722, Republic of Korea.
5Department of Laboratory Medicine, College of Medicine, Hanyang University, Seoul, 04763, Republic of Korea.
6Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, 04763, Republic of Korea.
*Corresponding author
Abstract
Understanding the interactions between nanoparticles (NPs) and human immune cells is necessary for justifying their utilization in consumer products and biomedical applications. However, conventional assays may be insufficient in describing the complexity and heterogeneity of cell–NP interactions. Herein, mass cytometry and single‐cell RNA‐sequencing (scRNA‐seq) are complementarily used to investigate the heterogeneous interactions between silver nanoparticles (AgNPs) and primary immune cells. Mass cytometry reveals the heterogeneous biodistribution of the positively charged polyethylenimine‐coated AgNPs in various cell types and finds that monocytes and B cells have higher association with the AgNPs than other populations. scRNA‐seq data of these two cell types demonstrate that each type has distinct responses to AgNP treatment: NRF2‐mediated oxidative stress is confined to B cells, whereas monocytes show Fcγ‐mediated phagocytosis. Besides the between‐population heterogeneity, analysis of single‐cell dose–response relationships further reveals within‐population diversity for the B cells and naïve CD4+ T cells. Distinct subsets having different levels of cellular responses with respect to their cellular AgNP doses are found. This study demonstrates that the complementary use of mass cytometry and scRNA‐seq is helpful for gaining in‐depth knowledge on the heterogeneous interactions between immune cells and NPs and can be incorporated into future toxicity assessments of nanomaterials.
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