한빛사 논문
Na Yeon Kim1,5, Sangkyu Lee2,5,*, Jeonghye Yu1, Nury Kim2, Seong Su Won2, Hyerim Park1 and Won Do Heo1,2,3,4,*
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. 2Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of Korea. 3KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. 4Cancer Metastasis Control Center, KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. 5These authors contributed equally: Na Yeon Kim, Sangkyu Lee.
*Correspondence to Sangkyu Lee or Won Do Heo.
Abstract
Despite efforts to visualize the spatio–temporal dynamics of single messenger RNAs, the ability to precisely control their function has lagged. This study presents an optogenetic approach for manipulating the localization and translation of specific mRNAs by trapping them in clusters. This clustering greatly amplified reporter signals, enabling endogenous RNA–protein interactions to be clearly visualized in single cells. Functionally, this sequestration reduced the ability of mRNAs to access ribosomes, markedly attenuating protein synthesis. A spatio–temporally resolved analysis indicated that sequestration of endogenous β-actin mRNA attenuated cell motility through the regulation of focal-adhesion dynamics. These results suggest a mechanism highlighting the indispensable role of newly synthesized β-actin protein for efficient cell migration. This platform may be broadly applicable for use in investigating the spatio–temporal activities of specific mRNAs in various biological processes.
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TOP52020년 후보
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