한빛사 논문
대구경북첨단의료산업진흥재단
Nam-Young Kanga,b,‡, JungYeol Leec,‡, Sang Hee Leed,e, In Ho Songd, Yong Hwa Hwangf, Min Jun Kimf, Wut Hmone Phuea, Bikram Keshari Agrawallag, Si Yan Diana Wana, Janise Lalica, Sung-Jin Parka, Jong-Jin Kimh, Haw-Young Kwonh, So Hee Imi, Myung Ae Baei, Jin Hee Ahnj, Chang Siang Limk, Adrian Kee Keong Teok,m, Sunyou Parkc, Sang Eun Kimd,e,l, Byung Chul Leed,l, Dong Yun Leef,*, Young-Tae Changa,h,n,*
aLaboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore 138667, Singapore
bDepartment of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea
cNew drug discovery center, DGMIF, Daegu 41061, Republic of Korea
dDepartment of Nuclear Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam, 13620, Republic of Korea
eDepartment of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea
f Department of Bioengineering, College of Engineering, and BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, and Institute of Nano Science & Technology (INST), Hanyang University, Seoul 04763, Republic of Korea
gDepartment of Chemistry, National University of Singapore, 117543, Singapore.
hCenter for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea
iBio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Yuseong-Gu, Gajeongro 141, Daejeon 34114, Republic of Korea
jDepartment of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea
kStem Cells and Diabetes Laboratory, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore 138673, Singapore
lCenter for Nanomolecular Imaging and Innovative Drug Development, Advanced Institutes of Convergence Technology, Suwon, 16229, Republic of Korea
mDepartment of Biochemistry and department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore
nDepartment of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea
*Corresponding Authors
Author Contributions
‡These authors contributed equally.
Abstract
Pancreatic β cells are responsible for insulin secretion and are important for glucose regulation in a healthy body and diabetic disease patient without prelabeling of islets. While the conventional biomarkers for diabetes have been glucose and insulin concentrations in the blood, the direct determination of the pancreatic β cell mass would provide critical information for the disease status and progression. By combining fluorination and diversity-oriented fluorescence library strategy, we have developed a multimodal pancreatic β cell probe PiF for both fluorescence and for PET (positron emission tomography). By simple tail vein injection, PiF stains pancreatic β cells specifically and allows intraoperative fluorescent imaging of pancreatic islets. PiF-injected pancreatic tissue even facilitated an antibody-free islet analysis within 2 h, dramatically accelerating the day-long histological procedure without any fixing and dehydration step. Not only islets in the pancreas but also the low background of PiF in the liver allowed us to monitor the intraportal transplanted islets, which is the first in vivo visualization of transplanted human islets without a prelabeling of the islets. Finally, we could replace the built-in fluorine atom in PiF with radioactive 18F and successfully demonstrate in situ PET imaging for pancreatic islets.
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TOP52020년 후보
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