한빛사 논문
Mooseok Jang1,2,3,5,*, Hakseok Ko1,2,5, Jin Hee Hong1,2, Won Kyu Lee4, Jae-Seung Lee4 & Wonshik Choi1,2,*
1 Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science (IBS), 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea.
2 Department of Physics, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea.
3 Department of Bio and Brain Engineering, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea.
4 Department of Materials Science and Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Korea.
5 These authors contributed equally: Mooseok Jang, Hakseok Ko
*Correspondence to Mooseok Jang or Wonshik Choi
Abstract
To extend the imaging depth of high-resolution optical microscopy, various gating operations—confocal, coherence, and polarization gating—have been devised to filter out the multiply scattered wave. However, the imaging depth is still limited by the multiply scattered wave that bypasses the existing gating operations. Here, we present a space gating method, whose mechanism is independent of the existing methods and yet effective enough to complement them. Specifically, we reconstruct an image only using the ballistic wave that is acousto-optically modulated at the object plane. The space gating suppresses the multiply scattered wave by 10–100 times in a highly scattering medium, and thus enables visualization of the skeletal muscle fibers in whole-body zebrafish at 30 days post fertilization. The space gating will be an important addition to optical-resolution microscopy for achieving the ultimate imaging depth set by the detection limit of ballistic wave.
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