한빛사 논문
Wooyoung Kang1,6, Kook Sun Ha2,4,6, Heesoo Uhm1,5, Kyuhyong Park1, Ja Yil Lee3, Sungchul Hohng1,7,* & Changwon Kang2,7,*
1 Department of Physics and Astronomy, and Institute of Applied Physics, Seoul National University, Seoul 08826, Republic of Korea.
2 Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
3 School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea.
4 Present address: Department of Life Science, University of Suwon, Gyeonggi-do 18323, Republic
of Korea.
5 Present address: Department of Physics, University of Oxford, Oxford OX1 3PU, UK.
6 These authors contributed equally: Wooyoung Kang, Kook Sun Ha.
7 These authors jointly supervised this work: Sungchul Hohng, Changwon Kang.
*Correspondence to Sungchul Hohng or Changwon Kang
Abstract
Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time.
논문정보
TOP52020년 후보
관련 링크
연구자 키워드
관련분야 연구자보기
관련분야 논문보기
해당논문 저자보기