한빛사 논문
Sungsoo Kim1,7, Taeyoon Kyung2,6,7, Jae-Hee Chung1, Nury Kim2, Sehoon Keum2, Jinsu Lee1, Hyerim Park1, Ho Min Kim3,4, Sangkyu Lee2,*, Hee-Sup Shin2,* & Won Do Heo1,2,5,*
1 Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
2 Center for Cognition and Sociality, Institute for Basic Science (IBS), Daejeon, Republic of Korea.
3 Center for Biomolecular and Cellular Structure, Institute for Basic Science (IBS), Daejeon, Republic of Korea.
4 Graduate School of Medical Science & Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
5 KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
6 Present address: David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
7 These authors contributed equally: Sungsoo Kim, Taeyoon Kyung.
*Correspondence to Sangkyu Lee or Hee-Sup Shin or Won Do Heo
Abstract
Optogenetic approaches for controlling Ca2+ channels provide powerful means for modulating diverse Ca2+-specific biological events in space and time. However, blue light-responsive photoreceptors are, in principle, considered inadequate for deep tissue stimulation unless accompanied by optic fiber insertion. Here, we present an ultra-light-sensitive optogenetic Ca2+ modulator, named monSTIM1 encompassing engineered cryptochrome2 for manipulating Ca2+ signaling in the brain of awake mice through non-invasive light delivery. Activation of monSTIM1 in either excitatory neurons or astrocytes of mice brain is able to induce Ca2+-dependent gene expression without any mechanical damage in the brain. Furthermore, we demonstrate that non-invasive Ca2+ modulation in neurons can be sufficiently and effectively translated into changes in behavioral phenotypes of awake mice.
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TOP52020년 후보
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