한빛사 논문
Abstract
Tae-Wook Nam*, Ha Il Jung†,‡, Young Jun An†,‡, Young-Ha Park*, Sang Hee Lee‡, Yeong-Jae Seok*,§, and Sun-Shin Cha†,§
*Department of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 151-742, Korea; †Marine Biotechnology Center, Korea Ocean Research and Development Institute, Ansan 426-744, Korea; and ‡Department of Biological Sciences, Myongji University, Yongin 449-728, Korea
Edited by Winfried Boos, University of Konstanz, Konstanz, Germany, and accepted by the Editorial Board January 22, 2008 (received for review September 30, 2007)
Abstract
In Escherichia coli, glucose-dependent transcriptional induction of genes encoding a variety of sugar-metabolizing enzymes and transport systems is mediated by the phosphorylation state-dependent interaction of membrane-bound enzyme IICBGlc (EIICBGlc) with the global repressor Mlc. Here we report the crystal structure of a tetrameric Mlc in a complex with four molecules of enzyme IIBGlc (EIIB), the cytoplasmic domain of EIICBGlc. Each monomer of Mlc has one bound EIIB molecule, indicating the 1:1 stoichiometry. The detailed view of the interface, along with the high-resolution structure of EIIB containing a sulfate ion at the phosphorylation site, suggests that the phosphorylation-induced steric hindrance and disturbance of polar intermolecular interactions impede complex formation. Furthermore, we reveal that Mlc possesses a built-in flexibility for the structural adaptation to its target DNA and that interaction of Mlc with EIIB fused only to dimeric proteins resulted in the loss of its DNA binding ability, suggesting that flexibility of the Mlc structure is indispensable for its DNA binding.
enzyme IICBGlc | glucose signaling | protein-protein interaction | transcription regulation
Footnotes
Author contributions: T.-W.N., H.I.J. and Y.J.A. contributed equally to this work; Y.-J.S. and S.-S.C. designed research; T.-W.N., H.I.J., Y.J.A., and Y.-H.P. performed research; S.H.L. contributed new reagents/analytic tools; T.-W.N., Y.-J.S., and S.-S.C. analyzed data; and Y.-J.S. and S.-S.C. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission. W.B. is a guest editor invited by the Editorial Board.
§To whom correspondence may be addressed.
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
관련분야 논문보기
해당논문 저자보기