한빛사 논문
Kyoo Heo1, Young-Ha Park1, Kyung-Ah Lee2,3, Joonwon Kim4, Hyeong-In Ham1, Byung-Gee Kim4,5, Won-Jae Lee2,3 & Yeong-Jae Seok1,*
1 School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, Republic of Korea.
2 School of Biological Sciences and National Creative Research Initiative Center for Hologenomics, Seoul National University, Seoul 08826, Republic of Korea.
3 Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Republic of Korea.
4 School of Chemical and Biological Engineering and Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Republic of Korea.
5 Institute of Engineering Research, Seoul National University, Seoul 08826, Republic of Korea.
*Correspondence to Yeong-Jae Seok
Abstract
Biofilm formation protects bacteria from stresses including antibiotics and host immune responses. Carbon sources can modulate biofilm formation and host colonization in Vibrio cholerae, but the underlying mechanisms remain unclear. Here, we show that EIIAGlc, a component of the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS), regulates the intracellular concentration of the cyclic dinucleotide c-di-GMP, and thus biofilm formation. The availability of preferred sugars such as glucose affects EIIAGlc phosphorylation state, which in turn modulates the interaction of EIIAGlc with a c-di-GMP phosphodiesterase (hereafter referred to as PdeS). In a Drosophila model of V. cholerae infection, sugars in the host diet regulate gut colonization in a manner dependent on the PdeS-EIIAGlc interaction. Our results shed light into the mechanisms by which some nutrients regulate biofilm formation and host colonization.
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