한빛사 논문
Abstract
Eunjin Byeona,1, Jun Chul Parka,1, Atsushi Hagiwarab,c, Jeonghoon Hana,*, Jae-Seong Leea,*
a Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea
b Institute of Integrated Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan
c Organization for Marine Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan
1These authors equally contributed to this work.
*Corresponding authors : Jeonghoon Han, Jae-Seong Lee
Abstracts
To understand effects of two widely used antidepressant on the antioxidant defense mechanism in the marine rotifer Brachionus koreanus, we assessed acute toxicity and measured population growth, reactive oxygen species (ROS) levels, glutathione (GSH) levels, and antioxidant enzymatic activities (GST, GR, and SOD) in response to fluoxetine hydrochloride (FLX) and sertraline hydrochloride (SER). The no observed effect concentration-24 h of fluoxetine and sertraline were 1000 μg/L and 450 μg/L, respectively, whereas the median lethal concentration (LC50)-24 h of fluoxetine and sertraline were 1560 μg/L and 507 μg/L, respectively. Both fluoxetine and sertraline caused significant reduction in the population growth rate indicating that both antidepressants have a potential adverse effect on life cycle parameters of B. koreanus. The intracellular ROS level and GSH level were significantly modulated (P < 0.05) in response to fluoxetine and sertraline. In addition, antioxidant enzymatic activities have shown significant (P < 0.05) modulation in response to FLX and SER in B. koreanus. Furthermore, transcriptional profiles of antioxidant genes (GSTs, SODs, and GR) have shown modulation in response to FLX compared to SER-exposed B. koreanus. Our results indicate that fluoxetine and sertraline induce oxidative stress, leading to reduction in the population density and modulation of antioxidant defense mechanism in the marine rotifer B. koreanus.
Keywords : Selective serotonin reuptake inhibitors; oxidative stress; antioxidant system; glutathione S-transferase; rotifer
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