한빛사 논문
Suk Won Choi1,+, Moon Young Ryu1,+, András Viczián2,+, Hyun Ju Jung1, Gu Min Kim1, Agustin L. Arce3, Natalia P. Achkar3, Pablo Manavella3, Ulla Dolde4, Stephan Wenkel5, Attila Molnár6, Ferenc Nagy2, Seok Keun Cho1,*, Seong Wook Yang1,5,*
1 Department of Systems Biology, Institute of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, Korea
2 Institute of Plant Biology, Biological Research Centre (BRC), Hungarian Academy of Sciences, H-6726 Szeged, Temesvári krt. 62, Hungary
3 Instituto de Agrobiotecnología del Litoral (IAL) Centro Científico Tecnológico Santa Fe (CCT), Santa Fe, Argentina
4 Laboratoire de Recherche en Sciences Végétales, 24, chemin de Borde-Rouge.BP 42617 Auzeville. 31326, Castanet-Tolosan. FRANCE
5 Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Copenhagen, Denmark
6 Institute of Molecular Plant Sciences, School of Biological Sciences, The King’s Buildings, University of Edinburgh, Edinburgh, EH9 3BF, UK
+ These authors contributed equally to this work
*Corresponding author : Seok Keun Cho, Seong Wook Yang
Abstract
The shift of dark-grown seedlings into light causes enormous transcriptome changes followed by a dramatic developmental transition. Here, we show that miRNA biogenesis also undergoes regulatory changes during de-etiolation. Etiolated seedlings maintain low levels of primary-miRNAs (pri-miRNAs) and miRNA processing core proteins, such as Dicer-like 1 (DCL1), SERRATE (SE) and HYPONASTIC LEAVES 1 (HYL1), whereas during de-etiolation, both pri-miRNAs and the processing components accumulated to high levels. However, most miRNA levels did not notably increase in response to light. To reconcile this inconsistency, we demonstrate that an unknown suppressor decreases miRNA-processing activity and light-induced SMALL RNA DEGRADING NUCLEASE 1 (SDN1) shortens the half-life of several miRNAs in de-etiolated seedlings. Taken together, we suggest a novel mechanism, miRNA-biogenetic inconsistency, which accounts for the intricacy of miRNA biogenesis during de-etiolation. This mechanism is essential for the survival of de-etiolated seedlings after long-term skotomorphogenesis and their optimal adaptation to ever-changing light conditions.
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