한빛사 논문
Heinrich Heine University of Düsseldorf, Max Planck Institute for Plant Breeding Research
Joon-Seob Eom1,2,11, Dangping Luo3,11, Genelou Atienza-Grande4,10, Jungil Yang1,2, Chonghui Ji3, Van Thi Luu1,2, José C. Huguet-Tapia5, Si Nian Char6, Bo Liu3, Hanna Nguyen4, Sarah Maria Schmidt1,2, Boris Szurek7, Casiana Vera Cruz4, Frank F. White5, Ricardo Oliva4, Bing Yang3,8,* and Wolf B. Frommer1,2,9,*
1 Institute for Molecular Physiology and Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich Heine University of Düsseldorf, Düsseldorf, Germany.
2 Max Planck Institute for Plant Breeding Research, Cologne, Germany.
3 Division of Plant Sciences, Bond Life Sciences Center, University of Missouri, Columbia, MO, USA.
4 International Rice Research Institute, Metro Manila, Philippines.
5 Department of Plant Pathology, University of Florida, Gainesville, FL, USA.
6 Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USA.
7 IRD, CIRAD, Université Montpellier, IPME, Montpellier, France.
8 Donald Danforth Plant Science Center, St. Louis, MO, USA.
9 Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Aichi, Japan.
10 Present address: College of Agriculture and Food Science, University of the Philippines, Los Baños, Philippines.
11 These authors contributed equally: Joon-Seob Eom, Dangping Luo.
*Correspondence to Bing Yang or Wolf B. Frommer.
Abstract
Blight-resistant rice lines are the most effective solution for bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo). Key resistance mechanisms involve SWEET genes as susceptibility factors. Bacterial transcription activator-like (TAL) effectors bind to effector-binding elements (EBEs) in SWEET gene promoters and induce SWEET genes. EBE variants that cannot be recognized by TAL effectors abrogate induction, causing resistance. Here we describe a diagnostic kit to enable analysis of bacterial blight in the field and identification of suitable resistant lines. Specifically, we include a SWEET promoter database, RT–PCR primers for detecting SWEET induction, engineered reporter rice lines to visualize SWEET protein accumulation and knock-out rice lines to identify virulence mechanisms in bacterial isolates. We also developed CRISPR–Cas9 genome-edited Kitaake rice to evaluate the efficacy of EBE mutations in resistance, software to predict the optimal resistance gene set for a specific geographic region, and two resistant ‘mega’ rice lines that will empower farmers to plant lines that are most likely to resist rice blight.
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