한빛사 논문
Jing Zhang 1,2,3, Gugan Eswaran 1,2,11, Juan Alonso-Serra 1,2,11, Melis Kucukoglu1,2, Jiale Xiang 1,2, Weibing Yang3, Annakaisa Elo1,2, Kaisa Nieminen 4, Teddy Damén1,2, Je-Gun Joung5, Jae-Young Yun 6, Jung-Hun Lee7, Laura Ragni 8, Pierre Barbier de Reuille9, Sebastian E. Ahnert3,10, Ji-Young Lee 7,*, Ari Pekka Mähönen 1,2,* and Ykä Helariutta 1,2,3,*
1Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, Finland. 2Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland. 3The Sainsbury Laboratory, University of Cambridge, Cambridge, UK. 4Production Systems, Natural Resources Institute Finland (Luke), Helsinki, Finland. 5Samsung Genome Institute, Samsung Medical Center, Seoul, South Korea. 6Center for Genome Engineering, Institute for Basic Science, Daejeon, South Korea. 7School of Biological Sciences, Seoul National University, Seoul, South Korea. 8ZMBP-Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany. 9Institute of Plant Sciences, University of Bern, Bern, Switzerland. 10Theory of Condensed Matter, Cavendish Laboratory, University of Cambridge, Cambridge, UK. 11These authors contributed equally: G. Eswaran, J. Alonso-Serra.
*To whom correspondence should be addressed.
Abstract
Vascular cambium, a lateral plant meristem, is a central producer of woody biomass. Although a few transcription factors have been shown to regulate cambial activity1, the phenotypes of the corresponding loss-of-function mutants are relatively modest, highlighting our limited understanding of the underlying transcriptional regulation. Here, we use cambium cell-specific transcript profiling followed by a combination of transcription factor network and genetic analyses to identify 62 new transcription factor genotypes displaying an array of cambial phenotypes. This approach culminated in virtual loss of cambial activity when both WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNOTTED-like from Arabidopsis thaliana 1 (KNAT1; also known as BREVIPEDICELLUS) were mutated, thereby unlocking the genetic redundancy in the regulation of cambium development. We also identified transcription factors with dual functions in cambial cell proliferation and xylem differentiation, including WOX4, SHORT VEGETATIVE PHASE (SVP) and PETAL LOSS (PTL). Using the transcription factor network information, we combined overexpression of the cambial activator WOX4 and removal of the putative inhibitor PTL to engineer Arabidopsis for enhanced radial growth. This line also showed ectopic cambial activity, thus further highlighting the central roles of WOX4 and PTL in cambium development.
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