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조회 366  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Comparative analysis of isoflavone aglycones using microwave-assisted acid hydrolysis from soybean organs at different growth times and screening for their digestive enzyme inhibition and antioxidant properties

Du Hyun Kima, Won Tae Yanga, Kye Man Chob,*, Jin Hwan Leea,*

a Department of Life Resource Industry, Dong-A University, Busan 49315, Republic of Korea
b Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 34111, Republic of Korea

*Corresponding authors : Kye Man Cho, Jin Hwan Lee

Abstract
The objectives of this research were to demonstrate the changes in isoflavone-aglycones, total phenolics, and biological properties (digestive enzyme inhibition; antioxidant) from six organs including leaves, leafstalks, roots, stems, seeds, and pods at different growth times of soybean plant. Three isoflavone-aglycones in microwave-assisted acid hydrolysis extracts were elucidated using UHPLC-ESI-Q-TOF-MS/MS and their contents exhibited remarkable differences in leaves (245.93–2239.33 μg/g), roots (854.96–4425.34 μg/g), and seeds (ND–2339.62 μg/g). Specifically, the collected samples on 15-Oct (leaves: 2239.33; seeds: 2339.62 μg/g) and 31-Aug (roots: 4425.34 μg/g) showed the highest isoflavone-aglycones, and daidzein was observed the most abundant component, comprising approximately 70%. Moreover, the inhibitions against α-glucosidase and α-amylase displayed the predominant effects in roots (89;91%) and leaves (81;85%) of samples on 31-Aug and 15-Oct at 300 μg/ml. The antioxidant activities on ABTS, DPPH, and hydroxyl radicals increased considerably with the increases of growth times in leaves and seeds, especially, ABTS showed the highest scavenging abilities: leaves (15-Oct;83%) > roots (31-Aug;75%) > seeds (15-Oct;68%). Therefore, our results suggest that soybean leaves, roots and seeds may be considered as excellent natural sources for nutraceuticals.

Chemical compounds studied in this 
Daidzein (PubChem CID: 5281708); Glycitein (PubChem CID: 5317750); Genistein (PubChem CID: 5280961)

Keywords : Soybean organ; Isoflavone aglycone; Growth times; Antioxidant activity; Glucosidase; Amylase; Microwave-assisted acid hydrolysis

Abbreviations
ABTS, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid); DPPH, 2,2-diphenyl-1-pycrylhydrazyl; NMR, nuclear magnetic resonance; UPLC-ESI-Q-TOF-MS/MS, ultra high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry; HPLC, high performance liquid chromatography; DMSO, dimethyl sulfoxide

논문정보
- 형식: Research article
- 게재일: 2019년 09월 (BRIC 등록일 2019-09-26)
- 연구진: 국내연구진 태극기
- 분야: Food_Technology
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