한빛사 논문
Abstract
Alisha Wehdnesday Bernardo Reyes, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Son Hai Vu, Chang Keun Kang, Wongi Min, Hu Jang Lee, John Hwa Lee, Suk Kim*
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea
*Corresponding author : Suk Kim
Abstract
We investigated the involvement of chemokine receptor type 4 (CXCR4) signaling on the outcome of Brucella (B.) abortus 544 infection in murine macrophages and in a mouse model. CXCR4 manipulation were first evaluated for Brucella invasion and intracellular survival efficiency, mitogen-activated protein kinases (ERK1/2, JNK, p38α) activation and generation of nitric oxide (NO), and then in the splenic bacterial proliferation and cytokine production in BALB/c mice. CXCR4 blockade is involved in the successful control of Brucella invasion, reduction of ERK1/2 phosphorylation and inhibition of nitric oxide release from macrophages. Furthermore, using a reported CXCR4-specific antagonist AMD3100 resulted in splenomegaly but attenuated Brucella proliferation in these organs with elevated serum levels of MCP-1, TNF and IL-12. These findings provide insights on the contribution of CXCR4 signaling in the phagocytic pathway and immune modulation during B. abortus infection.
Keywords : AMD3100; B. abortus; Cytokines; CXCR4; Invasion; MAPKs
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