한빛사 논문
Heon Seok Kim1,2,3,7,8, You Kyeong Jeong1,2,8, Junho K Hur4,5, Jin-Soo Kim3,6,* and Sangsu Bae1,2,*
1 Department of Chemistry, Hanyang University, Seoul, South Korea.
2 Research Institute for Natural Sciences, Hanyang University, Seoul, South Korea.
3 Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea.
4 Department of Pathology, College of Medicine, Kyung Hee University, Seoul, South Korea.
5 Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, South Korea.
6 Department of Chemistry, Seoul National University, Seoul, South Korea.
7 Present address: Division of Oncology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA.
8 These authors contributed equally: Heon Seok Kim, You Kyeong Jeong.
*Correspondence to Jin-Soo Kim or Sangsu Bae.
Abstract
Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5–7) and in a confined TC*N sequence context. Adenine base editor–induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.
논문정보
TOP52019년 선정
관련 링크
연구자 키워드
관련분야 연구자보기
관련분야 논문보기