한빛사 논문
So Dam Ha1,5, Seokjin Ham2,5, Min Young Kim1,5, Ji Hyun Kim1, Insoon Jang3, Bo Bae Lee1, Min Kyung Lee1, Jin-Taek Hwang4, Tae-Young Roh2,3,* & TaeSoo Kim1,*
1 Department of Life Science and the Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 03760, Korea. 2 Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea. 3 Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea. 4 Korea Food Research Institute, Wanju 55365, Korea. 5 These authors equally contributed: So Dam Ha, Seokjin Ham, and Min Young Kim.
*Correspondence and requests for materials should be addressed to T.-Y.R. or to T.K.
Abstract
In yeast, Hda1 histone deacetylase complex (Hda1C) preferentially deacetylates histones H3 and H2B, and functionally interacts with Tup1 to repress transcription. However, previous studies identified global increases in histone H4 acetylation in cells lacking Hda1, a component of Hda1C. Here, we find that Hda1C binds to hyperactive genes, likely via the interaction between the Arb2 domain of Hda1 and RNA polymerase II. Additionally, we report that Hda1C specifically deacetylates H4, but not H3, at hyperactive genes to partially inhibit elongation. This role is contrast to that of the Set2–Rpd3S pathway deacetylating histones at infrequently transcribed genes. We also find that Hda1C deacetylates H3 at inactive genes to delay the kinetics of gene induction. Therefore, in addition to fine-tuning of transcriptional response via H3-specific deacetylation, Hda1C may modulate elongation by specifically deacetylating H4 at highly transcribed regions.
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