한빛사 논문
Abstract
Ji Won Lima, Tai-Yong Kima,b, Sung-Wook Choia, Min-Ah Wooa,*
a Korea Food Research Institution, Consumer Food Safety Research Department, Wanju-gun, Jeonrabuk-do 55365, South Korea
b Chonbuk National University, Department of Food Science and Technology, Jeonju-si, Jeollabuk-do 54896, South Korea
*Corresponding author : Min-Ah Woo
Abstract
A point-of-care testing chip was developed for the colorimetric detection of inorganic mercury ion (Hg 2+). The disposable chip fabricated by three-dimensional printing technology contains DNAzymes produced by rolling circle amplification (RCA); a color change caused by the enzymatic reaction between DNAzymes and the peroxidase substrate 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is measured using a portable spectrophotometer. In the “turn-off”-type RCA reaction, the annealing of the T(12) primer that initiates the RCA reaction is blocked by the interaction of thymine with Hg2+; thus, the amount of amplified DNAzymes causing a color change is decreased depending on Hg2+ concentration. The colorimetric signal is enhanced by amplifying double-repeat DNAzymes from a circular DNA template. The chip detected Hg2+ in tap drinking water samples with high sensitivity (lowest validated value: 3.6 μg/L) and showed better selectivity, precision, and reproducibility than conventional analysis instruments. This low-cost easy-to-use platform can reduce the risk of accidental Hg2+ poisoning.
Keywords : Point-of-care (POC) testing; Mercury; 3D-printing; DNAzyme; Colorimetric detection; Drinking Water
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