한빛사 논문
Moonseok Kim1,2,3,4,8, Yonghyeon Jo1,2,8, Jin Hee Hong1,2, Suhyun Kim5, Seokchan Yoon1,2, Kyung-Deok Song1,2, Sungsam Kang6, Byunghak Lee7, Guang Hoon Kim7, Hae-Chul Park5 & Wonshik Choi1,2,*
1 Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science, Seoul 02841, Korea. 2 Department of Physics, Korea University, Seoul 02841, Korea. 3 Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea. 4 Department of Biomedicine & Health Sciences, The Catholic University of Korea, Seoul 06591, Korea. 5 Department of Biomedical Sciences, Korea University, Ansan 425-707, Korea. 6 Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. 7 Korea Electrotechnology Research Institute, Ansan 15588, Korea. 8 These authors contributed equally: Moonseok Kim, Yonghyeon Jo.
*Correspondence to Wonshik Choi.
Abstract
Label-free in vivo imaging is crucial for elucidating the underlying mechanisms of many important biological systems in their most native states. However, the applicability of existing modalities has been limited to either superficial layers or early developmental stages due to tissue turbidity. Here, we report a synchronous angular scanning microscope for the rapid interferometric recording of the time-gated reflection matrix, which is a unique matrix characterizing full light-specimen interaction. By applying single scattering accumulation algorithm to the recorded matrix, we removed both high-order sample-induced aberrations and multiple scattering noise with the effective aberration correction speed of 10,000 modes/s. We demonstrated in vivo imaging of whole neural network throughout the hindbrain of the larval zebrafish at a matured stage where physical dissection used to be required for conventional imaging. Our method will expand the scope of applications for optical imaging, where fully non-invasive interrogation of living specimens is critical.
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