한빛사 논문
Do Yon Kim1,2, Su Bin Moon1,2, Yong-Sam Kim1,2,*
1 Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea
2 Department of Biomolecular Science, KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, Republic of Korea
*Correspondence : Yong-Sam Kim
Abstract
The unsurpassed efficiency of CRISPR technology helps to sustain animal health and welfare through reducing the number of animals sacrificed. CRISPR tools are also used to create animals that are genetically designed in a more sophisticated fashion. In vitro technologies should ideally be complemented by animal studies. We previously [1] claimed the benefits of refining guide RNAs in CRISPR technology and we, as researchers who are engaged in producing and using model mice on a daily basis, also support the welfare and health of animals. At the same time, we feel that it is a solemn obligation and the highest priority to ensure human health and welfare, hopefully with the aid of biotechnology. To begin, we hold that engineering CRISPR guide RNAs definitely advances these goals. Since the first gene-modified (GM) mouse with germline transmission was created in 1981 [2], technical progress has been made to produce various GM organisms by different methods including random mutagenesis, the PiggyBac system, Cre–lox recombination, and recent genome-editing technologies [3]. Among these genome-editing methods, CRISPR technology has revolutionized GM technology not only because of easy technical accessibility and low cost but also because of its unsurpassed efficiency. In contrast to a claim by Bailey [4], many studies have reported high indel efficiencies, some of them even over 80% 5., 6.. This implies that there is no need to use more mice in a single study than before. In our laboratory we recently studied 24 mouse pups born
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