한빛사 논문
부산대학교
Abstract
Junbeom Leea, Chan-Hee Kima, Ho Am Janga, Jiyeun Kate Kimb, Toyomi Kotakic, Tetsuro Shinodad, Tetsuro Shinadae, Jin-Wook Yooa, Bok Luel Leea,∗
a Global Research Laboratory, College of Pharmacy, Pusan National University, Busan, 46241, South Korea
b Department of Microbiology, College of Medicine, Kosin University, Busan, 49267, South Korea
c National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8634, Japan
d Faculty of Food and Agricultural Sciences Fukushima University, 1 Kanayagawa, Fukushima, 960-1248, Japan
e Graduate School of Science, Osaka City University, Osaka, 558-8585, Japan
*Corresponding author : Bok Luel Lee
Abstract
Recent studies have provided molecular evidence that gut symbiotic bacteria modulate host insect development, fitness and reproduction. However, the molecular mechanisms through which gut symbionts regulate these aspects of host physiology remain elusive. To address these questions, we prepared two different Riptortus-Burkholderia insect models, Burkholderia gut symbiont-colonized (Sym) Riptortus pedestris insects and gut symbiont-noncolonized (Apo) insects. Upon LC-MS analyses, juvenile hormone III skipped bisepoxide (JHSB3) was newly identified from Riptortus Apo- and Sym-female and male adults’ insect hemolymph and JHSB3 titer in the Apo- and Sym-female insects were measured because JH is important for regulating reproduction in adult insects. The JHSB3 titer in the Sym-females were consistently higher compared to those of Apo-females. Since previous studies reported that Riptortus hexamerin-α and vitellogenin proteins were upregulated by the topical abdominal application of a JH-analog, chemically synthesized JHSB3 was administered to Apo-females. As expected, the hexamerin-α and vitellogenin proteins were dramatically increased in the hemolymph of JHSB3-treated Apo-females, resulting in increased egg production compared to that in Sym-females. Taken together, these results demonstrate that colonization of Burkholderia gut symbiont in the host insect stimulates biosynthesis of the heteroptera-specific JHSB3, leading to larger number of eggs produced and enhanced fitness in Riptortus host insects.
Keywords : Symbiosis; Riptortus pedestris; Burkholderia; Juvenile hormone; JHSB3
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기