한빛사 논문
부경대학교
Abstract
David Nkurunzizaa, Phillip Pendletonb,*, Byung Soo Chuna,*
a Department of Food Science and Technology, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea
b School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5000, Australia
*Corresponding author : Phillip Pendleton, Byung Soo Chun
Abstract
In this study, the soybean milk and tofu byproduct okara was subjected to subcritical water extraction with the intention of recovering isoflavones with minimal degradation. Response Surface Methodology (RSM) of the extraction variables indicated that optimized conditions would be T = 146.23 °C, P = 3.98 MPa, and α = 20 mg (solid)/mL (extractant). Mathematical models for the conversion and degradation of isoflavones were solved as a set of simultaneous equations leading to rate constants and time-dependent concentration profiles for each genistein- and daidzein-based compound. These kinetic analyses suggested that an optimum extraction time, under RSM-optimized conditions, would be 213.5 ± 8.7 min. The results of our study suggest that okara byproducts could be valorized efficiently, as a source of bioactive isoflavone aglycones, using subcritical water. The mathematical models and optimized extraction conditions that we established in this study could be employed, as process control-optimized variables, in the exploitation of okara, specifically in the isolation of genistein and daidzein.
Keywords : Subcritical water; isoflavones; aglycones; glucosides; response surface methodology; kinetics modeling
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