한빛사 논문
So Yoen Choi1, Rodrigo Lopez-Gonzalez1,4, Gopinath Krishnan1,4, Hannah L. Phillips2, Alissa Nana Li3, William W. Seeley3, Wei-Dong Yao2, Sandra Almeida1 and Fen-Biao Gao1*
1 Department of Neurology, University of Massachusetts Medical School, Worcester, MA, USA. 2 Department of Psychiatry and Behavioral Sciences, SUNY Upstate Medical University, Syracuse, NY, USA. 3 Memory and Aging Center, Department of Neurology, University of California, San Francisco, CA, USA. 4 These authors contributed equally: Rodrigo Lopez-Gonzalez, Gopinath Krishnan
*Correspondence to Fen-Biao Gao
Abstract
The GGGGCC repeat expansion in C9ORF72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). However, it is not known which dysregulated molecular pathways are primarily responsible for disease initiation or progression. We established an inducible mouse model of poly(GR) toxicity in which (GR)80 gradually accumulates in cortical excitatory neurons. Low-level poly(GR) expression induced FTD/ALS-associated synaptic dysfunction and behavioral abnormalities, as well as age-dependent neuronal cell loss, microgliosis and DNA damage, probably caused in part by early defects in mitochondrial function. Poly(GR) bound preferentially to the mitochondrial complex V component ATP5A1 and enhanced its ubiquitination and degradation, consistent with reduced ATP5A1 protein level in both (GR)80 mouse neurons and patient brains. Moreover, inducing ectopic Atp5a1 expression in poly(GR)-expressing neurons or reducing poly(GR) level in adult mice after disease onset rescued poly(GR)-induced neurotoxicity. Thus, poly(GR)-induced mitochondrial defects are a major driver of disease initiation in C9ORF72-related ALS/FTD.
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