한빛사 논문
Minsuk Kwak†‡§∥◆ , Wonji Gu#∇◆, Heekyung Jeong#∇○, Hyunjung Lee†‡§, Jung-uk Lee#∇○, Minji An#∇, Yong Ho Kim∥⊥ , Jae-Hyun Lee#∇ , Jinwoo Cheon*#∇○ , and Young-wook Jun*†‡§#∇
† Department of Otolaryngology, University of California, San Francisco, San Francisco, California 94158, United States
‡ Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94158, United States
§ Helen Diller Family Comprehensive Cancer Center (HDFCCC), University of California, San Francisco, San Francisco, California 94158, United States
∥ SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 16419, Republic of Korea
⊥ Department of Chemistry, Sungkyunkwan University, Suwon 16419, Republic of Korea
# Center for Nanomedicine, Institute for Basic Science (IBS), Seoul 03722, Republic of Korea
∇ Yonsei-IBS Institute, Yonsei University, Seoul 03722, Republic of Korea
○ Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea
*Corresponding Authors
Author Contributions
◆These authors contributed equally.
Abstract
Multifunctional magnetic nanoparticles have shown great promise as next-generation imaging and perturbation probes for deciphering molecular and cellular processes. As a consequence of multicomponent integration into a single nanosystem, pre-existing nanoprobes are typically large and show limited access to biological targets present in a crowded microenvironment. Here, we apply organic-phase surface PEGylation, click chemistry, and charge-based valency discrimination principles to develop compact, modular, and monovalent magnetofluorescent nanoparticles (MFNs). We show that MFNs exhibit highly efficient labeling to target receptors present in cells with a dense and thick glycocalyx layer. We use these MFNs to interrogate the E-cadherin-mediated adherens junction formation and F-actin polymerization in a three-dimensional space, demonstrating the utility as modular and versatile mechanogenetic probes in the most demanding single-cell perturbation applications.
Keywords: cell labeling; cell surface microenvironment; Magnetic nanoparticles; single-cell perturbation biology; steric crowding
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