한빛사 논문
Jae-Hoon Ji1,*, Sunwoo Min2, Sunyoung Chae3, Geun-Hyoung Ha4, Yonghyeon Kim2, Yeon-Ji Park2, Chang-Woo Lee4 and Hyeseong Cho1,2,*
1 Genomic Instability Research Center, Ajou University School of Medicine, Suwon, South Korea, 2 Department of Biochemistry and Molecular Biology, Ajou University School of Medicine, Suwon, South Korea, 3 Institute of Medical Science, Ajou University School of Medicine, Suwon, South Korea and 4 Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, South Korea
*To whom correspondence should be addressed.
Correspondence may also be addressed to Hyeseong Cho.
Abstract
Histone H2AX undergoes a phosphorylation switch from pTyr142 (H2AX-pY142) to pSer139 (γH2AX) in the DNA damage response (DDR); however, the functional role of H2AX-pY142 remains elusive. Here, we report a new layer of regulation involving transcription-coupled H2AX-pY142 in the DDR. We found that constitutive H2AX-pY142 generated by Williams-Beuren syndrome transcription factor (WSTF) interacts with RNA polymerase II (RNAPII) and is associated with RNAPII-mediated active transcription in proliferating cells. Also, removal of pre-existing H2AX-pY142 by ATM-dependent EYA1/3 phosphatases disrupts this association and requires for transcriptional silencing at transcribed active damage sites. The following recovery of H2AX-pY142 via translocation of WSTF to DNA lesions facilitates transcription-coupled homologous recombination (TC-HR) in the G1 phase, whereby RAD51 loading, but not RPA32, utilizes RNAPII-dependent active RNA transcripts as donor templates. We propose that the WSTF-H2AX-RNAPII axis regulates transcription and TC-HR repair to maintain genome integrity.
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