한빛사 논문
Jinah Janga,1, Hun-Jun Parkb,1, Seok-Won Kima, Heejin Kimb, Ju Young Parkc, Soo Jin Nab, Hyeon Ji Kima, Moon Nyeo Parka, Seung Hyun Choid, Sun Hwa Parke, Sung Won Kime, Sang-Mo Kwond, Pum-Joon Kimb,*, Dong-Woo Choa,*
a Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea
b Division of Cardiovascular Medicine, The Catholic University of Korea, College of Medicine, Cardiovascular Center, Seoul St. Mary's Hospital, 222 Banpodaero, Seocho-gu, Seoul, 06591, South Korea
c Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Kyungbuk, 37673, South Korea
d Medical Research Institute, Laboratory for Vascular Medicine and Stem Cell Biology, Department of Physiology, School of Medicine, Convergence Stem Cell Research Center, Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Pusan National University, 20 Geumo-ro, Mulgeum-eup, Yangsan, 50612, South Korea
e Department of Otolaryngology-Head and Neck Surgery, Department of Biomedical Science, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591, South Korea
1J.J and H.-J.P were contributed equally to this work.
*Corresponding authors
Abstract
Stem cell therapy is a promising therapeutic method for the treatment of ischemic heart diseases; however, some challenges prohibit the efficacy after cell delivery due to hostile microenvironment of the injured myocardium. 3D printed pre-vascularized stem cell patch can enhance the therapeutic efficacy for cardiac repair through promotion of rapid vascularization after patch transplantation. In this study, stem cell-laden decellularized extracellular matrix bioinks are used in 3D printing of pre-vascularized and functional multi-material structures. The printed structure composed of spatial patterning of dual stem cells improves cell-to-cell interactions and differentiation capability and promotes functionality for tissue regeneration. The developed stem cell patch promoted strong vascularization and tissue matrix formation in vivo. The patterned patch exhibited enhanced cardiac functions, reduced cardiac hypertrophy and fibrosis, increased migration from patch to the infarct area, neo-muscle and capillary formation along with improvements in cardiac functions. Therefore, pre-vascularized stem cell patch provides cardiac niche-like microenvironment, resulting in beneficial effects on cardiac repair.
Keywords : 3D printing; Hydrogel; Decellularized extracellular matrix; Stem cells; Tissue engineering
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