한빛사 논문
Jaewoo Yoon1, Mina Yasumoto-Hirose2,† , Hiroaki Kasai3
1 College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu 42601, Republic of Korea
2 Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan
3 School of Marine Biosciences, Kitasato University, 160-4 Utou, Okirai, Sanriku-cho, Ofunato, Iwate 022-0101, Japan
† Present address: Tropical Technology Plus, 12-75 Suzaki, Uruma, Okinawa 904-2234, Japan.
*Correspondence: Jaewoo Yoon
Abstract
A Gram-stain-negative, rod-shaped, non-motile, strictly aerobic, chemoheterotrophic, light-yellow-pigmented bacterium, designated strain 02OK1/10-76T, was isolated from a mangrove estuary in Japan by use of an in situ cultivation technique. Preliminary analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (96.9 %) to Frondibacter aureus A5Q-67 T. The DNA G+C content of strain 02OK1/10-76T was 35 mol%; MK-6 was the only menaquinone; and iso-C15 : 0, iso-C17 : 0 3-OH and iso-C16 : 0 3-OH were the major (>10 %) cellular fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, three unidentified aminolipids, an unidentified aminophospholipid and an unidentified lipid. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the strain represents a novel species of the genus Frondibacter , for which the name Frondibacter mangrovi sp. nov. is proposed. The type strain is 02OK1/10-76T (= KCTC 52666 T = NBRC 112695T). An emended description of F. aureus is also provided.
Keyword(s): 16S rRNA gene, polyphasic taxonomy, Bacteroidetes, Frondibacter mangrovi sp. nov., in situ cultivation, Flavobacteriaceae
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