한빛사 논문
Hyun Mu Shina,b,c,d, Youngjun Jue, Gwanghun Kima,b,c, Jae Won Leeb, Myung Won Seoa, Ji Hyun Sima, Jusang Yange, Sora Nohe, Jungbae Kime,*, Hang‐Rae Kima,b,c,d,*
aDepartment of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
bDepartment of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
cBK21Plus Biomedical Science Project, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
dMedical Research Institute, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
eDepartment of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea
H.M.S. and Y.J. contributed equally to this work.
*To whom correspondence should be addressed.
Abstract
The current practice of cytokine‐based immunotherapy relies on high doses and multiple injections of cytokine agents, which raises patients' inconvenience and economic burden. Here, sustainable and recyclable cytokine delivery based on short and injectable polymer fibers immobilized with interleukin (IL)‐2 and IL‐15 is demonstrated, which can be retained at the target tumor sites upon injection. In detail, electrospun polylactic acid (PLA) fibers are treated with aqueous ethanol solution for their dispersion, increasing the interfiber space for highly efficient biomolecule conjugation, and further immobilized with protein G via enzymatic dopamine coating. The protein G‐immobilized PLA fibers are cut into short fibers using a microtome, and filtering is performed to collect injectable short PLA (sPLA) fibers with the lengths of 15–100 µm. These sPLA fibers are further loaded with cytokines via the interaction between protein G and Fc, and cytokine‐loaded sPLA (Cyto‐sPLA) fibers are injected near the tumor sites using a syringe. The administration of Cyto‐sPLA fibers efficiently suppresses the tumor growth up to 70% by reinvigorating nonfunctional T cells to a functional state that can kill tumors in a sustainable and recyclable manner. The injectable sPLA‐fiber platform can be employed as a carrier for the efficient delivery of various agents in vivo.
Keywords : cancer immunotherapy, cytokine delivery, electrospun fibers, enzymatic polydopamine coating, injectable fibers
논문정보
관련 링크
연구자 키워드
관련분야 연구자보기
관련분야 논문보기
해당논문 저자보기