한빛사 논문
Abstract
Md Tawheed Hasana,b,1, Won Je Janga,1, Bong-Joo Leec, Kang Woong Kimc, Sang Woo Hurc, Sang Gu Limc, Sungchul C. Baid,*, In-Soo Konga,*
a Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea
b Department of Aquaculture, Sylhet Agricultural University, Sylhet, 3100, Bangladesh
c Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea
d Department of Marine Bio-Materials and Aquaculture, Pukyong National University, Busan, 608-737, Republic of Korea
*Corresponding author : Sungchul C. Bai, In-Soo Kong
1Equal contribution.
Abstract
Investigations were carried out to evaluate and quantify the effects of dietary supplementation with heat-killed (HK) Bacillus sp. SJ-10 (BSJ-10) probiotic (1 × 108 CFU g−1) on the growth and immunity of olive flounder (Paralichthys olivaceus). Flounder (averagely 9.64 g) were divided into two groups, and fed control and HK BSJ-10 (HKBSJ-10)-inoculated diets for 8 weeks. Investigations were carried out on growth and feed utilizations, innate immunity, serum biochemical parameters, microvilli length, and pro- and anti-inflammatory cytokine gene (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, and IL-10) transcriptions. Compared to control, HKBSJ-10 diet significantly (P < 0.05) enhanced weight gain and protein efficiency ratio, 1.17 and 1.11 folds respectively. Humoral innate immune parameters, lysozyme and superoxide dismutase in treatment group were also elevated by 1.34 and 1.16 folds. Similarly, an increased (P < 0.05) relative expressions of TNF-α, IL-1β, IL-6 were recorded in liver (2.71, 3.38, and 4.12 folds respectively), and gill (2.08, 1.98, and 1.81 folds respectively) than that of controls. Moreover, after challenge with Streptococcus iniae (1 × 108 CFU mL−1), the HKBSJ-10-fed group exhibited significantly higher protection (P < 0.05) against streptococcosis compared to controls, validating the observed changes in immune parameters and induction on the cytokine-encoding genes. Therefore, HKBSJ-10 increases growth, modulates innate immune parameters, and protects olive flounders against streptococcosis.
Keywords : HKBSJ-10; Growth performance; Innate immunity; Gene expression; Olive flounder
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