한빛사 논문
Ki-Baek Jeong†§, Ke Luo†§, Hwankyu Lee‡ , Min-Cheol Lim∇, Jin Yu⊥, Soo-Jin Choi⊥ , Ki-Bum Kim# , Tae-Joon Jeon∥ , and Young-Rok Kim*†
† Graduate School of Biotechnology and Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea
‡ Department of Chemical Engineering, Dankook University, Yongin 16891, Republic of Korea
∇ Research Group of Food Safety, Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do 55365, Republic of Korea
⊥ Department of Applied Food System, Major of Food Science & Technology, Seoul Women’s University, Seoul 01797, Republic of Korea
# Department of Materials Science and Engineering, Seoul National University, Seoul 08826, Republic of Korea
∥ Department of Biological Engineering, Inha University, Incheon 22212, Republic of Korea
*Corresponding Author
Author Contributions
§K.-B.J. and K.L. contributed equally to this work.
Abstract
Various types of biological and synthetic nanopores have been developed and utilized for the high-throughput investigation of individual biomolecules. Biological nanopores made with channel proteins are so far superior to solid-state ones in terms of sensitivity and reproducibility. However, the performance of a biological nanopore is dependent on the protein in the channel structure its dimensions are predetermined and are difficult to modify for broader applications. Here inspired by the cytotoxic mechanisms of a saponin derivative, alpha-hederin, we report a nonproteinaceous nanopore that can be formed spontaneously in a lipid membrane. We propose the pore-forming mechanism of alpha-hederin in a cholesterol-rich lipid membrane and a strategy to control the pore-forming rate by a lipid partitioning method. The small diameter and effective thickness of alpha-hederin nanopores enabled us to discriminate ssDNA homopolymers as well as four types of nucleotides, showing its potential as a DNA sequencing tool.
Keywords: alpha-hederin; discrimination; nanopore; self-assembly; single nucleotide; size-tunable
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