한빛사 논문
Abstract
Heung Joo Yuka,1, Hyung Won Ryua,1, Doo-Young Kima, Mi Hyeon Parka, Woo Duck Seob, Seong Hun Jeongc,*, Sei-Ryang Oha,*
a Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheong-ju si, Chungcheongbuk-do 28116, Republic of Korea
b Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jeollabuk-do 55365, Republic of Korea
c Namhae Garlic Research Institute, Namhae, Gyeongnam 52430, Republic of Korea
1 These authors contributed equally to this work.
*Corresponding authors
Abstract
Spinach intake has long been highlighted globally because of its outstanding nutritional aspects. In this study, changes in flavonoids, a representative functional phytochemical group, were investigated by UPLC-QTof MS with multivariate analysis of winter-spinach samples from three different cultivation regions in Korea. From the partial least squares discriminant analysis (PLS-DA), the differences of flavonoids among the geographic locations were clearly distinguished. Seven spinach flavonoids (2, patuletin-3-O-glucosyl-(1→6)-glucoside; 4, spinacetin-3-O-glucosyl-(1→6)-[apiosyl-(1→2)]-glucoside; 8, patuletin 3-O-(2″-feruloylglucosyl)-(1→6)-[apiosyl-(1→2)]-glucoside; 11, spinacetin 3-O-(2″-feruloylglucosyl)-(1→6)-[apiosyl-(1→2)]-glucoside; 12, patuletin 3-O-(2′'-feruloylglucosyl)-(1→6)-glucoside; 18, 5,3′,4′-trihydroxy 3-methoxy-6:7-methylendioxyflavone-4′-glucuronide; 20, 5,4′-dihydroxy-3,3′-dimethoxy-6:7-methylendioxyflavone-4′-glucuronide) were evaluated as key markers among 20 isolated metabolites. Interestingly, the contents of individual marker were significantly different among the groups, though total amount of flavonoids were almost same. Additionally, policosanols (PCs) in the winter-spinach was examined quantitatively using GC-MS for the first time. The PCs were analyzed as the range of 53.6-59.2mg/100g, indicate that the winter-spinach is a beneficial source of PCs.
Keywords : Spinacia oleraceaL., Spinach, Flavonoid, Policosanol, UPLC-QTof MS
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