한빛사 논문
Abstract
In Min Hwang1, Boyeon Park1, Yun Mi Dang, Su-Yeon Kim, and Hye Young Seo*
Hygienic Safety and Analysis Center, World Institute of Kimchi, Gwangju 61755, Republic of Korea
1 Bothauthors contributed equally to this paper.
* Corresponding author : Hye Young Seo
Abstract
Glucosinolates (GLS) have been reported to have significant anti-oxidative, antimicrobial, and anti-cancer activities. The current study was aimed to develop an analytical method for glucosinolate quantitation in eight Brassica species from Gwangju, Republic of Korea. For this purpose the UHPLC-Q-Orbitrap-MS technique was used and validated for optimal extraction conditions, detection and quantitation limits, linearity, precision, and accuracy. According to the results of GLS profiling, the total GLS concentration decreased in the order of cabbage > broccoli > cauliflower > mustard > Chinese cabbage > young radish ∼ kale. All Brassica species contained glucoerucin (GER) and glucobrassicin (GBR) as major GLS with the high levels in cabbage (5.913 μM/g) and broccoli (1.723 μM/g), respectively. The contents of minor GLS were species-dependent, and could therefore be used for Brassica species classification.
Keywords : Glucosinolate, Brassica, LC-ESI-MS, Validation
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