한빛사 논문
한양대학교
Abstract
Ji-Hye Kwona, Hyeon Gyu Leea, Kun-Ho Seob, Hyunsook Kima,*
aDepartment of Food and Nutrition, Hanyang University, Seoul 04763 , Korea
bKU Center for Food Safety, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea
J.-H. K. and H.G.L. contributed equally to this work.
*To whom correspondence should be addressed.
Abstract
Scope
This study aims to determine the separate and combined effect of a prebiotic (catechin-rich wine grape seed flour, GSF) and a probiotic (newly isolated kefir lactic acid bacteria, LAB) on hepatic steatosis of obese mice.
Methods and results
High-fat (HF)-induced obese mice are fed a HF diet containing 6% microcrystalline cellulose (MCC, control), 10% GSF, orally administrated LAB, or a combination of GSF and LAB for 9 weeks. There is a significant reduction of body weight gain and liver weights, plasma insulin concentrations, and HOMA-IR in all experimental groups compared to control. Total lipid content, triglyceride, and low-density lipoprotein (LDL)-cholesterol concentrations of the liver are also significantly lowered. The combination of GSF and LAB further significantly affects cecum propionate content, plasma aspartate aminotransferase (AST/GOT), and zonulin concentrations, which is significantly correlated with hepatic lipid content. Analysis of hepatic microarray data reveals that genes related to lipid synthesis, bile acid and cholesterol synthesis, antioxidant activities, oxidative stress, inflammation, and liver function are significantly modulated following the combination of GSF and LAB.
Conclusion
These data suggest that amelioration of HF-induced hepatic steatosis after consumption of GSF and LAB is partially mediated via alteration of cecum propionate and intestinal permeability, which modulates hepatic gene expression.
Keywords: grape seed flour, hepatic steatosis, lactic acid bacteria, prebiotics, probiotics
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