한빛사 논문
Hyomin Lee*†, Jihye Choi‡, Euihwan Jeong§∥, Seongho Baek‡, Hee Chan Kim⊥, Jong-Hee Chae#, Youngil Koh∇, Sang Woo Seo○, Jin-Soo Kim*§∥, and Sung Jae Kim*‡◆¶
†Department of Chemical and Biological Engineering, Jeju National University, Jeju, 63243, Republic of Korea
‡Department of Electrical and Computer Engineering and §Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea
∥Center for Genome Engineering, Institute for Basic Science, Seoul 34047, Republic of Korea
⊥Department of Biomedical Engineering and #Department of Pediatrics, Seoul National University Hospital, Seoul 03080, Republic of Korea
∇Department of Internal Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea
○School of Chemical and Biological Engineering, Institute of Chemical Process and ◆Nano System Institute, Seoul National University, Seoul 08826, Republic of Korea
¶ Inter-university Semiconductor Research Center, Seoul National University, Seoul 08826, Republic of Korea
*Corresponding Authors
H.L., J.C., and E.J. contributed equally.
Abstract
The-state-of-the-art bio- and nanotechnology have opened up an avenue to noninvasive liquid biopsy for identifying diseases from biomolecules in bloodstream, especially DNA. In this work, we combined sequence-specific-labeling scheme using mutated clustered regularly interspaced short palindromic repeats associated protein 9 without endonuclease activity (CRISPR/dCas9) and ion concentration polarization (ICP) phenomenon as a mechanism to selectively preconcentrate targeted DNA molecules for rapid and direct detection. Theoretical analysis on ICP phenomenon figured out a critical mobility, elucidating two distinguishable concentrating behaviors near a nanojunction, a stacking and a propagating behavior. Through the modulation of the critical mobility to shift those behaviors, the C-C chemokine receptor type 5 (CCR5) sequences were optically detected without PCR amplification. Conclusively, the proposed dCas9-mediated genetic detection methodology based on ICP would provide rapid and accurate micro/nanofluidic platform of liquid biopsies for disease diagnostics.
Keywords: dCas9; direct detection; Ion concentration polarization; liquid biopsy; micro/nanofluidics; selective preconcentration
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
관련분야 논문보기