[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보  |  e브릭몰e브릭몰 sale 회원가입   로그인
BRIC홈 농수식품
국내 바이오 소재 활용 촉진을 위한 설문조사
스폰서배너광고 안내  배너1 배너2 배너3 배너4
과학으로 본 코로나19 (COVID-19)
전체보기 농림성과 식품성과 인터뷰
윤새길
윤새길 (Saekil Yun) 저자 이메일 보기
서울대학교
저자CV 보기
182 KB
 
조회 471  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Immunostimulation of Cyprinus carpio using phage lysate of Aeromonas hydrophila

Saekil Yuna, Jin Woo Junb, Sib Sankar Giria, Hyoun Joong Kima, Cheng Chic, Sang Geun Kima, Sang Wha Kima, Jung Woo Kanga, Se Jin Hana, Jun Kwona, Woo Taek Oha, Se Chang Parka,*

a
Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea
b Department of Aquaculture, Korea National College of Agriculture and Fisheries, Jeonju, 54874, Republic of Korea
c Laboratory of Aquatic Nutrition and Ecology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

* Corresponding author: Se Chang Park, DVM, Ph.D.
Mailing address: College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, 81-417, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea 08826

Abstract
Over the last 50 years, various approaches have been established for the development of antigens for immunostimulation. We used phage lysate (PL), composed of inactivated antigens by the lytic bacteriophage pAh 6-c for Aeromonas hydrophila JUNAH strain to develop a vaccine for the prevention of A. hydrophila infection in Cyprinus carpio (common carp). We also assessed the poly D,L lactide-co-glycolic acid (PLGA) microparticles encapsulation method to increase the efficiency of the vaccine. Six groups of vaccines involving encapsulated by PLGA, formalin killed cells, or phage lysate at low or high concentration were prepared for intraperitoneal injection in C. carpio. Blood specimens and head kidney samples were collected at various time points for bacterial agglutination assay and to assess relative expression of immune-related genes interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), lysozyme C, and serum amyloid A (SAA). The vaccine groups using high dose phage lysate antigen showed significantly higher agglutination titers than all other groups at 4- and 6-weeks post vaccination (wpv), with the titer of the PLGA encapsulated vaccine group being highest from 10 wpv to the end of the experiment. The survival rate of fish immunized with the phage lysate vaccines were higher than that of fish immunized with the formailin killed cells vaccine in the challenge experiment conducted 6 wpv. Additionally, the PLGA-encapsulated high dose phage lysate antigen vaccinated groups showed the best protective efficacy in the challenge experiment 12 wpv. Vaccines using the phage lysate antigen also showed higher IL-1β and lysozyme C gene expression at 7 days post vaccination (dpv) and 2 wpv, and higher TNF-α gene expression was seen at 7 dpv. Higher SAA gene expression was seen in these groups at 1 dpv. These results suggest that phage lysate antigen has the potential to induce robust immune responses than formalin killed cells-based vaccines, and could be more effective as a novel inactivated antigen in preventing A. hydrophila infection in C. carpio.

Keywords : Inactivated antigen; Phage lysate; Aeromonas hydrophila; Poly D,L lactide-co-glycolic acid (PLGA); Cyprinus carpio

논문정보
- 형식: Research article
- 게재일: 2018년 12월 (BRIC 등록일 2018-12-07)
- 연구진: 국내(교신)+국외 연구진 태극기
- 분야: Immunology, Marine_Biology, Veterinary_Science
  댓글 0
등록
 
목록
가천대학교 가천융합의과학원
외부관련링크
Google scholar (by Saekil Yun)
Pubmed (by Saekil Yun)
프리미엄 Bio일정 Bio일정 프리미엄 안내
DGIST 뉴바이올로지전공 온라인 전공 연구활동 설명회 개최
DGIST 뉴바이올로지전공 온라인 전공 연구활동 설명회 개최
사전접수: ~2020.10.29
날짜: 2020.10.29
Online 개최
[2020 노벨상 특집 웨비나] 유전자 가위 기술의 최근 특허 & 생명윤리 이슈들
[고용보험가입자(재직자)] GLP 및 신뢰성보증 이해과정
[고용보험가입자(재직자)] GLP 및 신뢰성보증 이해과정
날짜: 2020.11.11~13
장소: 경기도 안양시 동안구 시민대로401 대륭테크노타운15차 901호 이레테크데이터랩스
[10x Genomics Webinar] Interrogating the Immunology of Infectious Disease with Single Cell Technologies
[고용보험가입자(재직자)] FDA Process Validation을 위한 통계처리 과정(미니탭실습, 고용보험미가입자 참여불가)
[고용보험가입자(재직자)] FDA Process Validation을 위한 통계처리 과정(미니탭실습, 고용보험미가입자 참여불가)
날짜: 2020.11.04~06
장소: 경기도 안양시 동안구 시민대로 401 대륭테크노타운15차 9층, 이레테크데이터랩스
제15회 KSGCT 정기학술대회/온라인
제15회 KSGCT 정기학술대회/온라인
초록접수: ~2020.10.08
사전접수: ~2020.10.23
날짜: 2020.11.05~06
Online 개최
 
위로가기
농수식품 홈  |  농수식품 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북   유튜브 유튜브    RSS서비스 RSS
에펜도르프코리아 광고