한빛사 논문
Jason N. Belling†§∥#, Joshua A. Jackman†#, Saziye Yorulmaz Avsar†, Jae Hyeon Park†, Yan Wang†, Michael G. Potroz†, Abdul Rahim Ferhan†, Paul S. Weiss§∥⊥, and Nam-Joon Cho*†‡
†School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore
‡School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive 637459, Singapore
§California NanoSystems Institute, ∥Department of Chemistry and Biochemistry, and ⊥Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States
*Corresponding Author
Author Contributions
#J.N.B. and J.A.J. contributed equally to this work.
Abstract
With mounting evidence that nanomaterials can trigger adverse innate immune responses such as complement activation, there is increasing attention to the development of strategies that mask the complement-activating properties of nanomaterials. The current gold standard to reduce complement activation of nanomaterials is the covalent attachment of polymer coatings on nanomaterial surfaces, even though this strategy provides only moderate protection against complement activation. Akin to protein coronas that form on nanomaterial surfaces in physiological fluids, noncovalent strategies based on protein adsorption would offer a simplified, biomimetic approach to mitigate complement activation. Herein, we demonstrate that precoating graphene-based nanomaterials with purified, natural proteins enables regulatory control of nanomaterial-triggered complement activation. When the graphene-based nanomaterials were coated with complement factor H, nearly complete protection (>90% reduction) against complement activation (a “stealth effect”) was achieved. By contrast, coating the nanomaterials with a passivating layer of bovine or human serum albumins achieved moderate protection (∼40% reduction), whereas immunoglobulin G amplified complement activation by several-fold. Taken together, our results demonstrate that surface-bound factor H, as well as serum albumins, can prevent graphene oxide-triggered complement activation, thereby offering a facile approach to inhibit complement activation completely down to naturally occurring levels.
Keywords: complement activation; factor H; graphene oxide; immunomodulation; protein coating; protein corona; serum albumin
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기