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A Phenome-Based Functional Analysis of Transcription Factors in the Cereal Head Blight Fungus, Fusarium graminearum
 Authors and Affiliations  Authors and Affiliations
Hokyoung Son1,†, Young-Su Seo1,†,‡, Kyunghun Min1, Ae Ran Park1, Jungkwan Lee2, Jian-Ming Jin1, Yang Lin1, Peijian Cao3, Sae-Yeon Hong1, Eun-Kyung Kim1, Seung-Ho Lee1, Aram Cho1, Seunghoon Lee1, Myung-Gu Kim1, Yongsoo Kim1, Jung-Eun Kim1, Jin-Cheol Kim4, Gyung Ja Choi4, Sung-Hwan Yun5, Jae Yun Lim1, Minkyun Kim1, Yong-Hwan Lee1, Yang-Do Choi1, Yin-Won Lee1*
1 Department of Agricultural Biotechnology and Centers for Fungal Pathogenesis and Agricultural Biomaterials, Seoul National University, Seoul, Korea, 2 Department of Applied Biology, Dong-A University, Busan, Korea, 3 China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute, Zhengzhou, Henan, China, 4 Chemical Biotechnology Center, Korea Research Institute of Chemical Technology, Daejon, Korea, 5 Department of Medical Biotechnology, Soonchunhyang University, Asan, Korea
* To whom correspondence may be addressed.
‡Current address: Department of Microbiology, Pusan National University, Busan, Korea
†These authors contributed equally to this work.
Abstract
Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins that are harmful to animal and human. In this study, a systematic analysis of 17 phenotypes of the mutants in 657 Fusarium graminearum genes encoding putative transcription factors (TFs) resulted in a database of over 11,000 phenotypes (phenome). This database provides comprehensive insights into how this cereal pathogen of global significance regulates traits important for growth, development, stress response, pathogenesis, and toxin production and how transcriptional regulations of these traits are interconnected. In-depth analysis of TFs involved in sexual development revealed that mutations causing defects in perithecia development frequently affect multiple other phenotypes, and the TFs associated with sexual development tend to be highly conserved in the fungal kingdom. Besides providing many new insights into understanding the function of F. graminearum TFs, this mutant library and phenome will be a valuable resource for characterizing the gene expression network in this fungus and serve as a reference for studying how different fungi have evolved to control various cellular processes at the transcriptional level.
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