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김도형 (Do-Hyoung Kim)  |
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광주과학기술원, University of California, Riverside |
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Endocrine regulation of airway clearance in Drosophila
 Authors and Affiliations
 Authors and Affiliations
Do-Hyoung Kima,b, Young-Joon Kimb, and Michael E. Adamsa,c,1
aDepartment of Entomology, University of California, Riverside, CA 92521; bSchool of Life Sciences, Gwangju Institute of Science and Technology, 61005 Gwangju, South Korea; and cDepartment of Cell Biology & Neuroscience, University of California, Riverside, CA 92521
1To whom correspondence should be addressed.
Abstract Fluid clearance from the respiratory system during developmental transitions is critically important for achieving optimal gas exchange in animals. During insect development from embryo to adult, airway clearance occurs episodically each time the molt is completed by performance of the ecdysis sequence, coordinated by a peptide-signaling cascade initiated by ecdysis-triggering hormone (ETH). We find that the neuropeptide Kinin (also known as Drosokinin or Leukokinin) is required for normal respiratory fluid clearance or “tracheal air-filling” in Drosophila larvae. Disruption of Kinin signaling leads to defective air-filling during all larval stages. Such defects are observed upon ablation or electrical silencing of Kinin neurons, as well as RNA silencing of the Kinin gene or the ETH receptor in Kinin neurons, indicating that ETH targets Kinin neurons to promote tracheal air-filling. A Kinin receptor mutant fly line (Lkrf02594) also exhibits tracheal air-filling defects in all larval stages. Targeted Kinin receptor silencing in tracheal epithelial cells using breathless or pickpocket (ppk) drivers compromises tracheal air-filling. On the other hand, promotion of Kinin signaling in vivo through peptide injection or Kinin neuron activation through Drosophila TrpA1 (dTrpA1) expression induces premature tracheal collapse and air-filling. Moreover, direct exposure of tracheal epithelial cells in vitro to Kinin leads to calcium mobilization in tracheal epithelial cells. Our findings strongly implicate the neuropeptide Kinin as an important regulator of airway clearance via intracellular calcium mobilization in tracheal epithelial cells of Drosophila.
Kinin | ecdysis-triggering hormone | tracheal collapse | tracheal air-filling | pickpocket
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논문정보 |
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- 형식: Research article - 게재일: 2018년 01월 (BRIC 등록일 2018-02-02) - 연구진: 국내+국외 연구진 - 분야: Developmental_Biology
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관련 인터뷰 |
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1. 논문관련 분야의 소개, 동향, 전망을 설명, 연구과정에서 생긴 에피소드
산소를 체내로 운반하고 이산화탄소를 배출하는 과정인 호흡은 생물이 가지고 있는 가장 기본적인 대사과정 중의 하나입니다. 곤충은 기관(Trachea)를 통하여 호흡을 하는데 기관의 기능과 발생과정은 인간의 폐와 유사점을 가지고 있습니다. 최근의 게놈연구결과, 기관이 분화하고 형성되는 과정에 관여하는 많은 유전자들이 인간의 폐형성에 관여한다는 것이 발견되어서 인간의 유전병을 이해하는데... |
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의약품 GMP 품질관리 실무
사전접수: ~2019.12.13
날짜: 2019.12.12~13
장소: 충북 청주시 흥덕구 오송읍 오송생명 1로 194-41 기업연구관 1관 충북산학융합본부 1층 강의실 |
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