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조회 781  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Gram-positive bacteria produce membrane vesicles: Proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles

Eun-Young Lee1,†, Do-Young Choi2,†, Dae-Kyum Kim1, Jung-Wook Kim1, Jung Ok Park2, Sungjee Kim3, Sang-Hyun Kim4, Dominic M. Desiderio5, Yoon-Keun Kim1, Kwang-Pyo Kim2,* and Yong Song Gho1,*

1
Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea
2 Institute of Biomedical Science and Technology, Department of Molecular Biotechnology, Konkuk University, Seoul, Republic of Korea
3 Department of Chemistry, Pohang University of Science and Technology, Pohang, Republic of Korea
4 The National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Republic of Korea
5 Department of Neurology, University of Tennessee Health Science Center, Memphis, TN, USA

These authors contributed equally to this work

*Corresponding authors

Abstract
Although archaea, Gram-negative bacteria, and mammalian cells constitutively secrete membrane vesicles (MVs) as a mechanism for cell-free intercellular communication, this cellular process has been overlooked in Gram-positive bacteria. Here, we found for the first time that Gram-positive bacteria naturally produce MVs into the extracellular milieu. Further characterizations showed that the density and size of Staphylococcus aureus-derived MVs are both similar to those of Gram-negative bacteria. With a proteomics approach, we identified with high confidence a total of 90 protein components of S. aureus-derived MVs. In the group of identified proteins, the highly enriched extracellular proteins suggested that a specific sorting mechanism for vesicular proteins exists. We also identified proteins that facilitate the transfer of proteins to other bacteria, as well to eliminate competing organisms, antibiotic resistance, pathological functions in systemic infections, and MV biogenesis. Taken together, these observations suggest that the secretion of MVs is an evolutionally conserved, universal process that occurs from simple organisms to complex multicellular organisms. This information will help us not only to elucidate the biogenesis and functions of MVs, but also to develop therapeutic tools for vaccines, diagnosis, and antibiotics effective against pathogenic strains of Gram-positive bacteria.

Keywords: Gram-positive bacteria; IgG-binding protein; Membrane vesicles; Microbiology; Microvesicles; Staphylococcus aureus

논문정보
- 형식: Research article
- 게재일: 2009년 12월 (BRIC 등록일 2017-03-27)
- 연구진: 국내(교신)+국외 연구진태극기
- 분야: Biochemistry, Microbiology
- 피인용횟수: 120회 이상 인용된 논문
Dot/Icm 제 4 유형 커플링 단백질 복합체의 선택적 이펙터 단백질 인식[Nat. Commun.]
김현민
발표: 김현민 (KAIST)
일자: 2020년 9월 29일 (화) 오후 02시 (한국시간)
언어: 한국어
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