Kyoungmi Kim1,3, Seuk-Min Ryu1-3, Sang-Tae Kim1, Gayoung Baek1, Daesik Kim2, Kayeong Lim1,2, Eugene Chung1,2, Sunghyun Kim1,2 & Jin-Soo Kim1,2
1Center for Genome Engineering, Institute for Basic Science, Seoul, Republic of Korea. 2Department of Chemistry, Seoul National University, Seoul, Republic of Korea. 3These authors contributed equally to this work.
Correspondence to : Jin-Soo Kim
Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.