한빛사 논문
Abstract
Bikram Keshari Agrawalla†, Hyo Won Lee§, Wut-Hmone Phue†, Anandhkumar Raju‡, Jong-Jin Kim†, Hwan Myung Kim§, Nam-Young Kang*‡, and Young-Tae Chang*†‡
† Department of Chemistry and Medicinal Chemistry Program, National University of Singapore, 3 Science Drive 3, 117543 Singapore
‡ Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, # 02-02 Helios, 138667 Singapore
§ Department of Energy Systems Research, Ajou University, Suwon 443749, Korea
*Corresponding Author
Abstract
Insulin-secreting beta cells together with glucagon-producing alpha cells play an essential role in maintaining the optimal blood glucose level in the body, so the development of selective probes for imaging of these cell types in live islets is highly desired. Herein we report the development of a 2-glucosamine-based two-photon fluorescent probe, TP-β, that is suitable for imaging of beta cells in live pancreatic islets from mice. Flow cytometry studies confirmed that TP-β is suitable for isolation of primary beta cells. Moreover, two-photon imaging of TP-β-stained pancreatic islets showed brightly stained beta cells in live islets. Insulin enzyme-linked immunosorbent assays revealed that TP-β has no effect on glucose-stimulated insulin secretion from the stained islet. Finally, to develop a more convenient islet imaging application, we combined our recently published alpha-cell-selective probe TP-α with TP-β to make a “TP islet cocktail”. This unique dye cocktail enabled single excitation (820 nm) and simultaneous dual-color imaging of alpha cells (green) and beta cells (red) in live pancreatic islets. This robust TP islet cocktail may serve as a valuable tool for basic diabetic studies.
논문정보
관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기