Seunghyun Kang1¶, Do-Hwan Ahn1¶, Jun Hyuck Lee1,2, Sung Gu Lee1,2, Seung Chul Shin1, Jungeun Lee1, Gi-Sik Min3, Hyoungseok Lee1, Hyun-Woo Kim4*, Sanghee Kim5* & Hyun Park1,2*
1Unit of Polar Genomics, Korea Polar Research Institute, Yeonsu-gu, Incheon, South Korea
2Polar Sciences, University of Science & Technology, Yuseong-gu, Daejeon, South Korea
3Department of Biological Sciences, Inha University, Incheon, South Korea
4Department of Marine Biology, Pukyong National University, Busan, South Korea
5Division of Polar Life Sciences, Korea Polar Research Institute, Yeonsu-gu, Incheon, South Korea
¶These authors contributed equally to this work.
*Corresponding authors : Hyun-Woo Kim, Sanghee Kim, Hyun Park
Background: The Antarctic intertidal zone is continuously subjected to extremely fluctuating biotic and abiotic stressors. The West Antarctic Peninsula is the most rapidly warming region on Earth. Organisms living in Antarctic intertidal pools are therefore interesting for research into evolutionary adaptation to extreme environments and the effects of climate change.
Findings: We report the whole genome sequence of the Antarctic-endemic harpacticoid copepod Tigriopus kingsejongensi. The 37 Gb raw DNA sequence was generated using the Illumina Miseq platform. Libraries were prepared with 65-fold coverage and a total length of 295 Mb. The final assembly consists of 48 368 contigs with an N50 contig length of 17.5 kb, and 27 823 scaffolds with an N50 contig length of 159.2 kb. A total of 12 772 coding genes were inferred using the MAKER annotation pipeline. Comparative genome analysis revealed that T. kingsejongensis-specific genes are enriched in transport and metabolism processes. Furthermore, rapidly evolving genes related to energy metabolism showed positive selection signatures.
Conclusions: The T. kingsejongensis genome provides an interesting example of an evolutionary strategy for Antarctic cold adaptation, and offers new genetic insights into Antarctic intertidal biota.
Keywords: Copepoda, Genome, Antarctic, Adaptation, Tigriopus