한빛사 논문
경상대학교, University of Illinois at Urbana-Champaign
Abstract
Gap-Don Kima, c, 1, Jin-Kyu Seob, 1, Hyeon-Woong Yumb, Jin-Yeon Jeongc, Han-Sul Yangb, c,*
a Department of Animal Sciences, University of Illinois at Urbana-Champaign, 1503 S Maryland Drive, Urbana, IL 61801, USA
b Division of Applied Life Science (BK21 plus), Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea
c Institute of Agriculture and Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju-si, Gyeongsangnam-do 52828, Republic of Korea
*Corresponding author
1Equal contributors.
Abstract
The purpose of this study was to find discrimination markers for four major meat species such as beef, pork, chicken and duck. Myofibrillar and sarcoplasmic proteins isolated from each meat type were analyzed by one-dimensional gel electrophoresis and some proteins were identified through LC-MS/MS analysis. We confirmed that troponin I (TnI), enolase 3, L-lactate dehydrogenase (LDH) and triose-phosphate isomerase (TPI) could be useful markers for discrimination of mammals from poultry due to their different electrophoretic mobility. Tropomyosin 1 and carbonic anhydrase 3 were observed as muscle fiber type-related proteins and these could also be markers to distinguish mammals from poultry. Species-specific peptides identified by LC-MS/MS spectra allow the identification of each species regardless of the same protein. Therefore, it is easy to discriminate between mammals and poultry by comparing the electrophoretic mobility of TnI, enolase 3, LDH, TPI and CA3, and each species could be identified through LC-MS/MS analysis.
Keywords : discrimination; meat species; protein marker; Gel electrophoresis; LC-MS/MS
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기